In simplistic terms, high affinity, high selectivity affinity ligands can be thought of as a lock-and-key model for biological recognition. IT’S ALL ABOUT THE FIT. Given the diversity across biologics, it is not possible to predict a priori which ligand type is better than others.  However, it is known that a single ligand class or scaffold will not work every time. The cost of time and failing to develop a solution is unrecoverable.

To avoid this, Avitide applies its proprietary portfolio of AVIPure™ ligand scaffolds (40+ to date) and library diversity (greater than 1E14), enabling a vast interrogation of different ligand sizes, shapes, conformations, and contact areas. The implementation of this extreme diversity enables Avitide’s delivery success rate and unrivaled affinity purification performance in the industry. Throughout ligand discovery stage, we quickly screen and arrive at optimal ligand candidates for each biomolecule focusing on affinity, stability, and manufacturability.

Following analytical characterization of ligand binding, elution kinetics, and selectivity, we deliver a data package to our partners and recommend lead ligands to transition to the resin development stage.

Affinity resin development

Once a subset of ligand candidates is selected, production is scaled up using methods appropriate for commercial resin manufacturing. We then produce hundreds of candidate resins for screening, evaluating a resin manufacturing DOE that takes into consideration ligands and ligand density, chemistry, matrix type (size and porosity), and linkers. We also evaluate affinity resin separation performance in relevant bioprocess feedstreams, establishing binding and elution conditions within each molecule’s stability profile and separation and operational conditions that achieve each of our partner’s resin specifications.